• Login
    View Item 
    •   Home
    • Student Scholarship
    • Honors Theses
    • View Item
    •   Home
    • Student Scholarship
    • Honors Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of SSDRCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Digital Repository Deposit Agreement

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Isolation And Characterization Mutants Defective In Cilia Regeneration In Chlamydomonas reindhartii

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Acheampong__20Ellen.doc
    Size:
    2.540Mb
    Format:
    Microsoft Word
    DownloadPDF Variant
    Thumbnail
    Name:
    auto_convert.pdf
    Size:
    779.2Kb
    Format:
    PDF
    Download
    Title
    Isolation And Characterization Mutants Defective In Cilia Regeneration In Chlamydomonas reindhartii
    Author
    Acheampong, Ellen
    Date
    May 2019
    
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/20.500.13013/687
    Abstract
    Cilia and flagella are identical brush-like organelles found on the surfaces of eukaryotic cells and are involved in motility, sensing, and signaling. Defects in cilia assembly or function lead to polycystic kidney disease, congenital heart disease, Bardet Biedl syndrome, and other emerging ciliopathies. In Chlamydomonas reinhardtii, one of the most well-studied flagella model organisms, regeneration of flagella to normal length and normal function occurs within 90 minutes of acid-shock deflagellation. During this process hundreds of genes are induced. Much is left to be determined about how cells regulate expression of these genes: How do cells detect the presence or absence of cilia? How do cells send an ‘absence of cilia’ signal to the nucleus? How are the hundreds of genes encoding cilia proteins coordinated with each other? Previous experiments found that cells that failed to upregulate a reporter of flagella gene expression also had a delay in flagella regeneration. The goals of this experiment were to generate new C. reinhardtii insertional mutants defective in cilia regeneration, to identify the mutated genes in these strains, and ultimately, to identify transcription factors and signaling components needed for flagella assembly. 3000 hygromycin-resistant colonies were generated by the insertion of aph7” DNA fragment through electroporation. 42 of the 3000 colonies exhibited defective flagella structure, defective motility, or delayed flagella regeneration; 14 of these 42 mutants had delay in regenerating their flagella. To identify the mutated genes, genomic DNA for a subset of mutant strains was extracted, and Polymerase Chain Reaction (PCR) reactions were set up using the Restriction Enzyme Site Directed Amplification polymerase chain reaction (RESDA PCR) protocol with primers specific for the aph7” insert combined with four different degenerate primers. DNA fragments from successful PCR reactions were gel purified and sequenced. Using this method, mutated genes were identified and characterized to identify the proteins encoded by these genes. Flagella regeneration and reporter gene assays were conducted to further characterize the phenotypes of two of the mutants originally identified as having a delay.
    Advisor
    Brown, Jason M.
    Department
    Biology
    Degree
    Bachelor of Science (BS)
    Collections
    Honors Theses
    Biology Honors Theses

    entitlement

     
    DSpace software (copyright © 2002 - 2025)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.