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    Analysis of Wheatgrass Endophytes

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    Title
    Analysis of Wheatgrass Endophytes
    Author
    Clapp, Kimberly
    Date
    May 17, 2014
    Subject
    endophytes
    wheatgrass
    metabolites
    
    Metadata
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    URI
    http://hdl.handle.net/20.500.13013/571
    Abstract
    Endophytes are of particular medical interest due to their production of antibiotics. Wheatgrass endophytes were analyzed using a combination of MIC, Gel electrophoresis, GCMS, and TLC. Cultures were successfully grown in gel agarose plates and in sabouraud dextrose broth. All colonies were observed using a dissection microscope. Colonies from the white samples seen on the seed (sabouraud dextrose agar), leaf (sabouraud dextrose agar), seed (coffee agar) and seed (agar) plates were gram stained, with gram negative rods were observed in all samples. Gram positive cocci we observed in the seed (agar) plate. Presence of a long chain methyl ester was observed from the GCMS analysis of samples extracted in methanol. An inhibitory effect was observed on the growth of E. coli and S. aureus after inoculating methanol extracted samples in broth.
    Advisor
    MacTaylor, Christine
    Department
    Chemistry and Physics
    Degree
    Bachelor of Science (BS)
    Collections
    Chemistry and Physics Honors Theses
    Honors Theses

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