Evaluating Template-Switch Mutations (TSM) in E.Coli After Treatment with Dexamethasone, an FDA-Approved Anti-Inflammatory Drug
dc.contributor.advisor | Laranjo, Laura | en_US |
dc.contributor.author | Hubisz, Leigha-Mae | |
dc.creator | Hubisz, Leigha-Mae | en_US |
dc.date.accessioned | 2023-07-27T18:26:50Z | |
dc.date.available | 2023-07-27T18:26:50Z | |
dc.date.issued | 2023-05-01 | en_US |
dc.identifier.uri | http://hdl.handle.net/20.500.13013/2973 | |
dc.description.abstract | This presentation will provide an overview of an experiment to discover potential side-effects of current FDA-approved drugs, Dexamethasone, that can cause mutations in DNA. Mutations in DNA can change the structure and function of cells. DNA can transform into non-β form structures that stall replication and cause genomic instability. Quasi-palindromes (QP) are imperfect inverted repeats of DNA sequences which can block the DNA replication fork during DNA synthesis. If the DNA replication fork is blocked by quasi-palindrome structures, DNA polymerase can use an alternative method to continue DNA replication, called “template-switching,” which results in a perfect palindrome – a perfect inverted repeat of DNA. There is limited research for template-switch mutagenesis which tests selected FDA-approved drugs to understand the effect of TSM. The goal of this research project is to investigate the cellular effects of Dexamethasone, an anti-inflammatory glucocorticoid, which prevents the release of DNA, in quasi-palindrome mutations using E. coli as the model organism. The aim is to understand the consequence of selected drugs in template-switching quasi-palindrome mutations to increase our knowledge of the potential side effects for current FDA-approved drugs. | en_US |
dc.title | Evaluating Template-Switch Mutations (TSM) in E.Coli After Treatment with Dexamethasone, an FDA-Approved Anti-Inflammatory Drug | en_US |
dc.type | Thesis | en_US |
dc.description.department | Biology | en_US |
dc.date.display | May 2023 | en_US |
dc.type.degree | Bachelor of Science (BS) | en_US |
dc.subject.keyword | DNA | en_US |
dc.subject.keyword | quasi-palindrome | en_US |
dc.subject.keyword | template-switch | en_US |