Investigating FDA-Approved Anti-Tumor Drugs for Effects on Template-Switch Mutagenesis (TSM) in E.coli

Abstract

DNA mutations have profound implications for human health. Among the multiple sources of DNA mutations, are secondary structures. Quasipalindrome sequences (QP) are imperfect inverted repeats capable of forming hairpin-like DNA secondary structures. These structures can perturb DNA replication, resulting in mutations, DNA damage, and chromosomal rearrangements. Previous work has shown that these mutations can be caused by the addition of FDA-approved drugs such as 5-azaC, AZT, and ciprofloxacin. Dr. Laranjo and Ms. Addorisio will discuss the results of investigating two additional FDA approved antitumor drugs, CPT-11 and Doxorubicin hydrochloride for their ability to affect template-switch mutagenesis.