• Characterization Of A New ODA3 Allele, ODA3-6, Defective In Assembly Of The Outer Dynein Arm-Docking Complex In Chlamydomonas Reinhardtii

      Mosley, Matthew; Montes-Berrueta, Daniela; Hou, Yuqing; Yang, Fan; Scarbrough, Chasity; Witman, George B.; Wirschell, Maureen; Brown, Jason (2017-03-14)
      We have used an insertional mutagenesis approach to generate new C. reinhardtii motility mutants. Of 56 mutants isolated, one is a new allele at the ODA3 locus, called oda3-6. Similar to the previously characterized oda3 alleles, oda3-6 has a slow-jerky swimming phenotype and reduced swimming speed. The oda3-6 mutant fails to assemble the outer dynein arm motor and outer dynein arm—docking complex (ODA-DC) in the ciliary axoneme due to an insertion in the 5’ end of the DCC1 gene, which encodes the DC1 subunit of the ODA-DC. Transformation of oda3-6 with the wild-type DCC1 gene rescues the mutant swimming phenotype and restores assembly of the ODA-DC and the outer dynein arm in the cilium. This is the first oda3 mutant to be characterized at the molecular level and is likely to be very useful for further analysis of DC1 function.
    • Characterization of Microplastic and Mesoplastic Debris in Sediments from Kamilo Beach and Kahuku Beach, Hawai'i

      Young, Alan M.; Elliott, James A. (2016-11-11)
      Sediment samples were collected from two Hawai'ian beaches, Kahuku Beach on O'ahu and Kamilo Beach on the Big Island of Hawai'i. A total of 48,988 large microplastic and small mesoplastic (0.5–8 mm) particles were handpicked from the samples and sorted into four size classes (0.5–1 mm, 1–2 mm, 2–4 mm, 4–8 mm) and nine color categories. For all sizes combined the most common plastic fragment color was white/transparent (71.8%) followed by blue (8.5%), green (7.5%), black/grey (7.3%), red/pink (2.6%), yellow (1.2%), orange (0.6%), brown (0.3%) and purple (0.2%). Color frequency distribution based on both numbers and mass of particles was not significantly different among the various size classes nor between the two beaches. White and black/grey resin pellets accounted for 11.3% of the particles collected from Kahuku Beach and 4.2% of the particles from Kamilo Beach. Plastic type based on Raman Spectrometer analysis of a small representative subsample indicated that most of the fragments were polyethylene and a few were polypropylene. DOI: http://dx.doi.org/10.1016/j.marpolbul.2016.11.009
    • Exploring Art & Science In K-12 Education

      Delissio, Lisa (2016-06-27)
      Keynote speech/workshop on Art/Science integration in K12 Education at the Wadsworth Museum Summer Teacher Institute. The talk explores three main benefits of STEAM in K12 classrooms: Close Looking/Observation, Time on Task, and Returns to Creativity. Includes results from original research by Lisa Delissio and Rebecca Rohloff.
    • Life History And Population Dynamics Of Green Crabs (Carcinus maenas)

      Young, Alan M.; Elliott, James A. (2019-12-31)
      Carcinus maenas (the “shore crab” or “European green crab”) is a very proficient invader (considered to be one of the world’s 100 worst invaders by the IUCN) due to its phenotypic plasticity, wide temperature and salinity tolerance, and an extensive omnivorous diet. Native to Atlantic Europe, it has established two well-studied nonindigenous populations in the northwestern Atlantic and northeastern Pacific and less-studied populations in Australia, Argentina and South Africa. Green crabs are eurythermal and euryhaline as adults, but they are limited to temperate coastlines due to more restrictive temperature requirements for breeding and larval development. They cannot tolerate wave-swept open shores so are found in wave-protected sheltered bays, estuaries and harbors. Carcinus maenas has been the subject of numerous papers, with over 1000 published in the past decade. This review provides an up-to-date account of the current published information on the life history and population dynamics of this very important species, including genetic differentiation, habitat preferences, physical parameter tolerances, reproduction and larval development, sizes of crabs, densities of populations, sex ratios, ecosystem dynamics and ecological impacts in the various established global populations of green crabs.
    • The 'Ideal' Climate Change Ph.D. Program

      Drobot, Sheldon D.; Porinchu, David F.; Arzayus, Krisa M.; Barber, Valerie A.; Smith, L. Micaela; Warren, Jeff M.; Delissio, Lisa (2003-01-01)
      The training of the next generation of climate-change researchers is of utmost importance as climate change and its associated impacts take on increasing local, regional, and global relevance. This report seeks to address this issue by highlighting aspects of a successful climate-change Ph.D. program; a program which seeks to balance traditional disciplinary training with exposure to the broader, interdisciplinary climate-change community.