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    Discovery and Annotation of Microbacterium foliorum phage Juicer and Gordonia rubripertincta phage GiKK.

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    Title
    Discovery and Annotation of Microbacterium foliorum phage Juicer and Gordonia rubripertincta phage GiKK.
    Author
    Sprenkle, Amy B.
    Azeni, Brahim
    Bilbo, Merry
    Cashins, Robyn
    Gonzalez, Cristina
    Migneault, Nick
    Panza, Mariah
    Trevino, Serjio
    Date
    May 2021
    Subject
    Actinobacteriophage genome annotation
    Bioinformatics
    Phage Discovery
    SEA-PHAGES
    
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    URI
    http://hdl.handle.net/20.500.13013/1439
    Abstract
    Salem State was fortunate to be able to run the Phage Discovery semester of SEA-PHAGES in person in the Fall of 2020 under COVID-19 compliant protocols. Two actinobacterial hosts were used; Microbacterium foliorum and Gordonia rubripertincta. Student enriched soil samples yielded a number of phages on both hosts. We were able to amplify and purify two unique phages on each host, and finally send both DNA samples for full genome sequencing at the University of Pittsburgh. Microbacterium phage Juicer is a lytic siphoviridae in cluster EA6 at 41099 base pairs in length and displays typical genome organization. Genome annotation is accomplished by comparison to several close relatives. Gordonia phage GiKK is a lytic siphoviridae in cluster CT at 47537 base pairs and also has typical synteny and close relatives to compare for annotation during the Bioinformatics semester of SEA-PHAGES at Salem State University.
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