• Login
    View Item 
    •   Home
    • Events
    • Research Day
    • Research Day 2020
    • Undergraduate Posters
    • View Item
    •   Home
    • Events
    • Research Day
    • Research Day 2020
    • Undergraduate Posters
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of SSDRCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Most Popular ItemsStatistics by CountryMost Popular Authors

    Anti-Cancer Drugs Effect on Quasi-palindrome Mutations in Escherichia coli

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    PerkinsSydney.pdf
    Size:
    368.7Kb
    Format:
    PDF
    Download
    Title
    Anti-Cancer Drugs Effect on Quasi-palindrome Mutations in Escherichia coli
    Author
    Perkins, Sydney
    Dwyer, Joseph
    Patten, Madison
    Laranjo, Laura
    Date
    May 4, 2020
    Subject
    Mutations
    Anti-cancer drugs
    Chromosomal Reporter
    E.coli
    
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/20.500.13013/1416
    Abstract
    Cancer is one of the world's largest health problems in today’s age. The Global Burden of Disease estimates that 10 million people died as a result of cancer per year (IHME). Although there are many treatments for different types of cancer, many of the drugs used are known to have severe side effects. These known consequences include hair loss, nausea, and, unfortunately, an increase in DNA mutation, which can trigger other classes of diseases. One class of mutations that have been studied and linked to a form of cancer is Quasi-palindrome template switch mutations. Quasi-palindromes are nearly perfect inverted repeats of nucleotides able to form DNA secondary structures. These Hairpins are an example of a secondary DNA structure made by DNA repeats and are known to block the DNA replication fork (VOINEAGU et al. 2008). Once DNA replication is blocked by these secondary DNA structures, the DNA replication fork needs to find a solution to continue the replication process. At some frequency, DNA polymerase (responsible for replicating the DNA) can use alternative DNA strands as templates to make more DNA. One alternative method is called “Template-switching”, and it results in a mutation that creates a perfect palindrome from a quasi-palindromic sequence. Previous work has linked anti-cancer drugs such as 5-azacytidine to an increase of QP mutations (Laranjo 2018). Therefore, I am interested in analyzing other anti-cancer drugs and their potential to cause QP mutations. Using an FDA approved drug library, with over 300 different drugs, we will select anti-cancer drugs to screen for QP mutators. We have constructed a QP reporter in E. coli that is specific for QP mutations (Laranjo 2017). To understand the effect of anti-cancer drugs during DNA replication is critical to give us an understanding of potential side effects for patients undergoing chemotherapy.
    Sponsor
    Laranjo, Laura
    Department
    Biology
    Collections
    Undergraduate Posters

    entitlement

     
    DSpace software (copyright © 2002 - 2023)  DuraSpace
    Quick Guide | Contact Us
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.